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bsr t7 5  (ATCC)


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    Structured Review

    ATCC bsr t7 5
    Bsr T7 5, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5184 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bsr+t7+5/BHK-21/pm41972306-244-8-22
    Average 99 stars, based on 5184 article reviews
    bsr t7 5 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Stable Transfection:

    Article Title: Secreted Nonstructural Protein 3 is a Pathogenic Determinant of Orbivirus.
    Article Snippet: BALB/c mice were purchased from Liaoning Changsheng iotechnology Co., Ltd. Merino sheep were purchased from Jilin ian’an Zhihua Breeding Co., Ltd. All animals were bred under pecific pathogen-free (SPF) conditions at Harbin Veterinary esearch Institute, Chinese Academy of Agricultural Sciences, nd all animal experiments were approved by the Committee 4 of 21 on the Ethics of Animal Experiments of the Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences (approval numbers: 240507-06-GR for AG129 mice, 250717-04-GR for BALB/c mice, and 251031-01-GR for Merino sheep). .. BHK-21 (baby hamster kidney cells, ATCC CCL-10, USA), BSR-T7/5 (a BHK-21-derived clone stably expressing T7 RNA polymerase), HEK-293T (human embryonic kidney cells, ATCC CRL-3216, USA), CPAE (bovine pulmonary artery endothelial cells, ATCC, CCL-209, USA), and MDOK (Madin-Darby ovine kidney cells, ATCC, CRL-1633, USA) were maintained in highglucose Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) supplemented with 8% heat-inactivated fetal bovine serum (FBS, F0193-500ML, Sigma), 2 m m L-glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin, and nonessential amino acids. ..

    Expressing:

    Article Title: Secreted Nonstructural Protein 3 is a Pathogenic Determinant of Orbivirus.
    Article Snippet: BALB/c mice were purchased from Liaoning Changsheng iotechnology Co., Ltd. Merino sheep were purchased from Jilin ian’an Zhihua Breeding Co., Ltd. All animals were bred under pecific pathogen-free (SPF) conditions at Harbin Veterinary esearch Institute, Chinese Academy of Agricultural Sciences, nd all animal experiments were approved by the Committee 4 of 21 on the Ethics of Animal Experiments of the Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences (approval numbers: 240507-06-GR for AG129 mice, 250717-04-GR for BALB/c mice, and 251031-01-GR for Merino sheep). .. BHK-21 (baby hamster kidney cells, ATCC CCL-10, USA), BSR-T7/5 (a BHK-21-derived clone stably expressing T7 RNA polymerase), HEK-293T (human embryonic kidney cells, ATCC CRL-3216, USA), CPAE (bovine pulmonary artery endothelial cells, ATCC, CCL-209, USA), and MDOK (Madin-Darby ovine kidney cells, ATCC, CRL-1633, USA) were maintained in highglucose Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) supplemented with 8% heat-inactivated fetal bovine serum (FBS, F0193-500ML, Sigma), 2 m m L-glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin, and nonessential amino acids. ..

    Modification:

    Article Title: Secreted Nonstructural Protein 3 is a Pathogenic Determinant of Orbivirus.
    Article Snippet: BALB/c mice were purchased from Liaoning Changsheng iotechnology Co., Ltd. Merino sheep were purchased from Jilin ian’an Zhihua Breeding Co., Ltd. All animals were bred under pecific pathogen-free (SPF) conditions at Harbin Veterinary esearch Institute, Chinese Academy of Agricultural Sciences, nd all animal experiments were approved by the Committee 4 of 21 on the Ethics of Animal Experiments of the Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences (approval numbers: 240507-06-GR for AG129 mice, 250717-04-GR for BALB/c mice, and 251031-01-GR for Merino sheep). .. BHK-21 (baby hamster kidney cells, ATCC CCL-10, USA), BSR-T7/5 (a BHK-21-derived clone stably expressing T7 RNA polymerase), HEK-293T (human embryonic kidney cells, ATCC CRL-3216, USA), CPAE (bovine pulmonary artery endothelial cells, ATCC, CCL-209, USA), and MDOK (Madin-Darby ovine kidney cells, ATCC, CRL-1633, USA) were maintained in highglucose Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) supplemented with 8% heat-inactivated fetal bovine serum (FBS, F0193-500ML, Sigma), 2 m m L-glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin, and nonessential amino acids. ..

    Chromatin Immunoprecipitation:

    Article Title: Oral dosing of the nucleoside analog obeldesivir is efficacious against RSV infection in African green monkeys
    Article Snippet: .. Therefore blinding was not a realistic option for this in-life portion of the studies. re p o rtfo lio | rep o rtin g su m m ary A pril2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Animals and other research organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research, and Sex and Gender in Research Laboratory animals Wild animals Reporting on sex Field-collected samples Ethics oversight Note that full information on the approval of the study protocol must also be provided in the manuscript. mouse anti-respiratory syncytial virus (RSV) monoclonal antibody directed against the Fusion protein (ascites), MAb858 mouse clone 133/1H (Millipore Sigma - MAB858-1) goat-anti-mouse-AlexaFluor647 (Invitrogen A-21235) Vendor (Sigma Millipore) validated use of the RSV antibody for detection of RSV by IF and ELISA HEp-2 (ATCC CCL-23) BSR-T7/5 (Karl-Klaus Conzelman - Pettenkofer Institut) HEp-2 cells were autheticated by the vendor. ..

    Flow Cytometry:

    Article Title: Oral dosing of the nucleoside analog obeldesivir is efficacious against RSV infection in African green monkeys
    Article Snippet: .. Therefore blinding was not a realistic option for this in-life portion of the studies. re p o rtfo lio | rep o rtin g su m m ary A pril2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Animals and other research organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research, and Sex and Gender in Research Laboratory animals Wild animals Reporting on sex Field-collected samples Ethics oversight Note that full information on the approval of the study protocol must also be provided in the manuscript. mouse anti-respiratory syncytial virus (RSV) monoclonal antibody directed against the Fusion protein (ascites), MAb858 mouse clone 133/1H (Millipore Sigma - MAB858-1) goat-anti-mouse-AlexaFluor647 (Invitrogen A-21235) Vendor (Sigma Millipore) validated use of the RSV antibody for detection of RSV by IF and ELISA HEp-2 (ATCC CCL-23) BSR-T7/5 (Karl-Klaus Conzelman - Pettenkofer Institut) HEp-2 cells were autheticated by the vendor. ..

    Magnetic Resonance Imaging:

    Article Title: Oral dosing of the nucleoside analog obeldesivir is efficacious against RSV infection in African green monkeys
    Article Snippet: .. Therefore blinding was not a realistic option for this in-life portion of the studies. re p o rtfo lio | rep o rtin g su m m ary A pril2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Animals and other research organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research, and Sex and Gender in Research Laboratory animals Wild animals Reporting on sex Field-collected samples Ethics oversight Note that full information on the approval of the study protocol must also be provided in the manuscript. mouse anti-respiratory syncytial virus (RSV) monoclonal antibody directed against the Fusion protein (ascites), MAb858 mouse clone 133/1H (Millipore Sigma - MAB858-1) goat-anti-mouse-AlexaFluor647 (Invitrogen A-21235) Vendor (Sigma Millipore) validated use of the RSV antibody for detection of RSV by IF and ELISA HEp-2 (ATCC CCL-23) BSR-T7/5 (Karl-Klaus Conzelman - Pettenkofer Institut) HEp-2 cells were autheticated by the vendor. ..

    Biomarker Discovery:

    Article Title: Oral dosing of the nucleoside analog obeldesivir is efficacious against RSV infection in African green monkeys
    Article Snippet: .. Therefore blinding was not a realistic option for this in-life portion of the studies. re p o rtfo lio | rep o rtin g su m m ary A pril2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Animals and other research organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research, and Sex and Gender in Research Laboratory animals Wild animals Reporting on sex Field-collected samples Ethics oversight Note that full information on the approval of the study protocol must also be provided in the manuscript. mouse anti-respiratory syncytial virus (RSV) monoclonal antibody directed against the Fusion protein (ascites), MAb858 mouse clone 133/1H (Millipore Sigma - MAB858-1) goat-anti-mouse-AlexaFluor647 (Invitrogen A-21235) Vendor (Sigma Millipore) validated use of the RSV antibody for detection of RSV by IF and ELISA HEp-2 (ATCC CCL-23) BSR-T7/5 (Karl-Klaus Conzelman - Pettenkofer Institut) HEp-2 cells were autheticated by the vendor. ..

    Virus:

    Article Title: Oral dosing of the nucleoside analog obeldesivir is efficacious against RSV infection in African green monkeys
    Article Snippet: .. Therefore blinding was not a realistic option for this in-life portion of the studies. re p o rtfo lio | rep o rtin g su m m ary A pril2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Animals and other research organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research, and Sex and Gender in Research Laboratory animals Wild animals Reporting on sex Field-collected samples Ethics oversight Note that full information on the approval of the study protocol must also be provided in the manuscript. mouse anti-respiratory syncytial virus (RSV) monoclonal antibody directed against the Fusion protein (ascites), MAb858 mouse clone 133/1H (Millipore Sigma - MAB858-1) goat-anti-mouse-AlexaFluor647 (Invitrogen A-21235) Vendor (Sigma Millipore) validated use of the RSV antibody for detection of RSV by IF and ELISA HEp-2 (ATCC CCL-23) BSR-T7/5 (Karl-Klaus Conzelman - Pettenkofer Institut) HEp-2 cells were autheticated by the vendor. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Oral dosing of the nucleoside analog obeldesivir is efficacious against RSV infection in African green monkeys
    Article Snippet: .. Therefore blinding was not a realistic option for this in-life portion of the studies. re p o rtfo lio | rep o rtin g su m m ary A pril2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Animals and other research organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research, and Sex and Gender in Research Laboratory animals Wild animals Reporting on sex Field-collected samples Ethics oversight Note that full information on the approval of the study protocol must also be provided in the manuscript. mouse anti-respiratory syncytial virus (RSV) monoclonal antibody directed against the Fusion protein (ascites), MAb858 mouse clone 133/1H (Millipore Sigma - MAB858-1) goat-anti-mouse-AlexaFluor647 (Invitrogen A-21235) Vendor (Sigma Millipore) validated use of the RSV antibody for detection of RSV by IF and ELISA HEp-2 (ATCC CCL-23) BSR-T7/5 (Karl-Klaus Conzelman - Pettenkofer Institut) HEp-2 cells were autheticated by the vendor. ..

    Cell Culture:

    Article Title: Pre-assembly of biomolecular condensate seeds drives RSV replication.
    Article Snippet: .. A549 (CCL-185, American Type Culture Collection (ATCC)), BSR T7/5 (ref. 55) (BHK-21 cells that constitutively express the T7 RNA polymerase, RRID:CVCL_RW96) and HEK293T cells (CRL-3216, ATCC) were cultured in DMEM (31966021, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS; F7524, Merck) and 1% penicillin–streptomycin (pen–strep; 15140122, Thermo Fisher Scientific). .. Vero cells (CCL-81, ATCC) were cultured in DMEM supplemented with 5% FBS and 1% pen–strep.

    Article Title: Pre-assembly of biomolecular condensate seeds drives RSV replication
    Article Snippet: .. A549 (ATCC, cat. no. CCL-185), BSR T7/5 (BHK-21 cells that constitutively express the T7 RNA polymerase) and HEK293T cells (ATCC, cat. no. CRL-3216) were cultured in DMEM (Thermo Fisher Scientific, cat. no. 31966021) supplemented with 10% fetal bovine serum (FBS) (Merck, cat. no. F7524) and 1% penicillin-Streptomycin (Pen-Strep) (Thermo Fisher Scientific, cat. no. 15140122). .. Vero cells (ATCC, cat. no. CCL-81) were cultured in DMEM supplemented with 5% FBS and 1% Pen-Strep.



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